July
24 – August 13, 2012
COLD
SPRING HARBOR LABORATORY
Application Deadline:
April 15, 2012
Arranged by:David
DiGregorio and Jack Waters
Advances in light
microscopy, digital image processing, and the
development of a variety of powerful fluorescent
probes present expanding opportunities for
investigating the nervous system, from synaptic
spines to networks in the brain. This intensive
laboratory and lecture course will provide
participants with the theoretical and practical
knowledge to utilize emerging imaging technologies.
The primary emphasis of the course will be on vital
light microscopy. Students will learn the principles
of light microscopy, as well as use of different
types of electronic cameras, laser scanning systems,
functional fluorophores, delivery techniques, and
digital image-processing software. In addition to
transmitted light microscopy for viewing cellular
motility, the course will examine a variety of
molecular probes of cell function, including
calcium-sensitive dyes, voltage-sensitive dyes,
photo-activated ("caged") compounds, and exocytosis
tracers. Issues arising in the combination of
imaging with electrophysiological methods will be
covered. Particular weight will be given to
multi-photon laser scanning microscopy and to newly
available biological fluorophores, especially
Green-Fluorescent Protein (GFP) and its variants. We
will use a spectrum of neural and cell biological
systems, including living animals, brain slices, and
cultured cells.
Applicants should have a strong background in the neurosciences or in cell biology. In their personal statements, applicants should specify: 1) their experience with optical techniques, 2) how they will apply optical methods in their current projects, 3) the microscope systems available to them, and 4) their long-term goals in learning more about optical methods.
More information can be found here.